Grant Instruments PCV-3000 - Manual (Page 6)

2. General Information
Multi-spin PCV-3000 is the next step of development of sms technology: it is a fully
automated device utilising the sms algorithm with good repeatability. Productivity of sms
algorithm using Multi-Spin is 10 times faster (up to 30 min per 1000 samples) than that for
centrifuge-vortex Combi-Spin.
Combi-Spin (the previous name Micro-Spin) was invented and published for the first time by
Biol. Dr. V.Bankovskis in 1989 and after the successful approbation and finishing patented in
1994 (V.K.Bankovskis et al., Riga, Latvia, Pat. No. P94-74).
Spin-mix-spin technology automates the repeated steps of spinning down of the sample
followed by vortex mixing. This cycle of repeats significantly reduces the likelihood of
contamination and therefore errors in PCR analysis as well as the overall time taken for
sample preparation.
PCR reaction preparation times have traditionally been lengthy, the Multi-Spin reduces this
time. Trying to achieve the same sms algorithm manually is around ten times slower (up to
30 min for 100 samples vs 30 min for 1000 samples on the Multi-Spin) and the reproducibility
of results may vary due to slight differences in the vortex and centrifugation stages, a normal
consequence of manual operations.
Page 6
Multi-spin PCV-3000
Operating Instructions
Table 1. Comparison of PCV-4200, PCV-3000 and PCV-6000
Specifications
Combispin
PCV-2400
Multi-Spin
PCV-3000
Multi-Spin
PCV -6000
Speed range max. 2800 rpm 3500 rpm 6000 rpm
RCF max. 700 x g 800 x g 2350 x g
Number of tubes
vortexing
1 individually 12 simultaneously
Time for completing “Spin- Mix -Spin” cycle:
for 2 microtubes 60 sec 25 sec 15 sec
for 12 microtubes 5 – 6 min 1 min 30 sec 1 min
for 100 microtubes 60 min 15 min 10 min
Version 2.02 - August 2012