2 introducing the nucleocounter scc-100 – ChemoMetec SCC-100 User Manual
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2 Introducing the NucleoCounter
9
2 Introducing the NucleoCounter SCC-100
The NucleoCounter method provides a fast alternative to the manual techniques,
yielding objective and reliable results. Furthermore the NucleoCounter technology
provides improved safety to the user compared to other comparable methods.
The NucleoCounter system is comprised of the NucleoCounter instrument for particle
analysis, SCC-Cassette for sample handling and the Lysis buffer for cell lysis (please
refer to appropriate Application Notes provided by ChemoMetec concerning lysing of
cells). Basics of the NucleoCounter system is explained on the next few pages.
The NucleoCounter system identifies and counts individual somatic cells containing
stained DNA. The sample pre-treatment is intended to allow the effective staining of
cellular DNA but it will also aid the dissolving of cell aggregates and reduce scatter from
fat globules and protein micelles present in the milk. Depending on the milk sample and
sample properties some additional effort might be needed to obtain reliable results
(please refer to appropriate Application Notes provided by ChemoMetec).
2.1
The NucleoCounter SCC-100 instrument
The NucleoCounter is shown in the figure below.
Figure 2. NucleoCounter instrument
Refer to chapter 5 with respect to the operation of the instrument.
Lid
Control Buttons
Keypad
Display
Weight 3 kg
Height 26
cm
Width 38
cm
Depth 22
cm