ChemoMetec NC-100 - Manual (Page 17)

1 Inspection and Unpacking of Equipment
1
Table of contents
DECLARATION OF CONFORMITY ................................................................................ VI
WEEE DIRECTIVE INFORMATION – EUROPE ONLY ...................................................... VIII
INTRODUCTION AND INTENDED USE .......................................................................... X
WARNINGS AND PRECAUTIONS ............................................................................... XII
1 INSPECTION AND UNPACKING OF EQUIPMENT ...................................................... 3
2 INTRODUCING THE NUCLEOCOUNTER ................................................................... 5
2.1 T HE N UCLEO C OUNTER INSTRUMENT ....................................................................... 5
2.1.1 Fluorescence microscope ............................................................................. 6
2.1.2 Built-in image analysis ................................................................................ 7
2.2 T HE N UCLEO C ASSETTE ...................................................................................... 7
2.2.1 Loading the cassette .................................................................................... 8
2.2.2 Fluorescent dye ........................................................................................... 8
2.2.3 Sample volume ............................................................................................ 8
2.2.4 Cassette handling ........................................................................................ 9
2.3 L YSIS BUFFER (R EAGENT A100) AND S TABILIZING BUFFER (R EAGENT B) ......................... 9
3 CONTROL BUTTONS, KEYPAD AND DISPLAY ......................................................... 11
3.1 I NTERACTIVE C ONTROLS ................................................................................... 11
3.1.1 Control Buttons .......................................................................................... 11
3.1.2 Keypad ...................................................................................................... 11
3.2 I NTERFACE D ISPLAY ........................................................................................ 12
3.2.1 Status indicator ......................................................................................... 12
3.2.2 Display screen ........................................................................................... 12
4 INSTALLATION AND START-UP ........................................................................... 13
4.1 P OWER ON , POWER OFF ................................................................................... 13
4.1.1 Starting up ................................................................................................. 13
4.1.2 Shutting down ........................................................................................... 14
4.2 I NSTALLATION OF N UCLEO V IEW .......................................................................... 14
5 OPERATION OF THE NUCLEOCOUNTER ................................................................. 15
5.1 R EADY MODE ................................................................................................ 15
5.2 I NSERTING AND REMOVING THE CASSETTE ............................................................... 15
5.2.1 Protection of the optical system ................................................................ 15
5.2.2 Inserting cassette ...................................................................................... 15
5.3 M EASURING - T HE R UN BUTTON ......................................................................... 16
5.3.1 Actuator movements ................................................................................. 16
5.3.2 Result presentation ................................................................................... 16
5.3.3 External computer ..................................................................................... 17
5.4 R ESULT DISPLAY ............................................................................................ 17
5.4.1 Result mode .............................................................................................. 17
5.4.2 Saving parameters ..................................................................................... 18