Dako Hybridizer User Manual
Page 21
Dako Hybridizer
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Handbook
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Display will confirm PGM number/name and Denat & Hyb times and temperatures. Cursor highlights
“Run PGM” line.
Press “Enter” button to accept.
The display prompts to “Add Slides and Close Lid”. Before adding slides insert two Humidity Control
Strips into the inside slide lid. After slides have been added saturate strips with distilled water or
equivalent (approx. 13 mL for dry strips) and close lid.
Cursor highlights “Start” line. Press “Enter” button to run the program.
(To return to the Main Menu, move the cursor to highlight “Main Menu” line and press “Enter” button).
Display indicates present temperature of the slides.
Once temperature reaches denaturation set point, Hybridizer will beep twice and denaturation time will
count down from the set time.
PGM 01 HER2
82°C :05; 45°C 20:00
Run PGM
Main Menu
PGM 01 HER2
Add Slides
-
Close Lid Start
Main Menu
PGM 01 HER2
**Heating**
Denat: 82°C :05
Present Temp: 42°C
PGM 01 HER2
Denat in Process
Denat: 82°C 02:28
Present Temp: 82°C