Bio-Rad Biofuel Enzyme Kit - Manual (Page 7)

Please read steps 5–7 fully before
proceeding. These steps are time sensitive!
5. Using the DPTP labeled with an “H”,
pipet 250 µl of 1.5 mM substrate into
your 15 ml conical tube containing
enzyme labeled “High Concentration
Enzyme”.
6. Using the DPTP labeled with an “L”, pipet
250 µl of 1.5 mM substrate into your
15 ml conical tube containing enzyme
labeled “Low Concentration Enzyme”.
START YOUR TIMER.
7. At the times indicated, use the correctly
labeled DPTP to remove 500 µl from the
15 ml conical tubes labeled “High
Concentration Enzyme” and “Low
Concentration Enzyme”, and add it to the
appropriately labeled cuvette that already
contains the stop solution.
8. Proceed with the analysis of your
samples. After you have finished your
analysis, rinse out reaction tubes,
cuvettes, and DPTPs with copious water
and save them for later activities.
Note: Do not discard unused stock solutions.
They will be used for the next activity.
32
Q
UICK GUIDE
Quick Guide
1.5 mM Substrate High concentration enzyme
1.5 mM Substrate Low concentration enzyme
High concentration
enzyme H1
1 min
H2
2 min
H3
8 min
Low concentration
enzyme L1
1 min
L2
2 min
L3
8 min