Bio-Rad Biofuel Enzyme Kit - Manual (Page 2)

Read and understand steps 8–11 fully
before proceeding. These steps are time
sensitive!
8. Using the DPTP labeled “C”, pipet 500 µl
of buffer into the 15 ml conical tube
labeled “Control” and gently mix. Once
you have mixed the buffer with the
substrate, remove 500 µl of this solution
and add it to your cuvette labeled “Start”.
9. Using the DPTP labeled “E”, pipet 1 ml of
enzyme into the 15 ml conical tube
labeled “Enzyme Reaction”. Gently mix,
then START YOUR TIMER.
10. At the times indicated, use the DPTP
labeled “E” to remove 500 µl of the
solution from the “Enzyme Reaction”
tube and add it to the appropriately
labeled cuvette containing the stop
solution.
11. After all the enzyme samples have been
collected, use the DPTP labeled “C” to
remove 500 µl of the solution from the
“Control” reaction tube and add it to the
cuvette labeled “End”.
12. Proceed with the analysis of your
samples. After you have finished your
analysis, rinse out your reaction (conical)
tubes, cuvettes, and DPTPs with copious
water and save them for later activities.
Note: Do not discard unused stock solutions.
They will be used for the next activity.
27
Quick Guide
Q
UICK GUIDE
Enzyme
reaction E1
1 min
E2
2 min
E3
4 min
E4
6 min
E5
8 min
Control End
8 min
Buffer Control
Enzyme Enzyme reaction
Control Start