Bio-Rad PV92 PCR Informatics Kit - Manual (Page 3)

Quick Guide
1. Label 1 screwcap tube containing 200 µl of
InstaGene matrix plus protease with your
initials.
2. Collect 2 hairs from yourself. Select hairs that
have either a noticeable sheath (a coating of
epithelial cells near the base of the hair), or a
good sized root (the bulb-shaped base of the
hair). Trim the hair, leaving the last ~2 cm of the
base of the hair. Place the trimmed hairs into
the screwcap tube with your initials.
3. Place your tube in the foam micro test-tube
holder and incubate at 56°C for 10 minutes in
a water bath. At the halfway point (5 minutes),
shake or vortex gently, then place it back in the
56°C water bath for the remaining 5 minutes.
4. Remove the tubes, gently shake or vortex, and
place them in a boiling water bath (100°C).
Incubate at 100°C for 5 minutes.
5. Remove the tubes from the boiling water bath
and shake or vortex the contents to resuspend.
Pellet the matrix by spinning at 6,000 x g for
5 minutes (or 2,000 x g for 10 minutes) in a
centrifuge.
6. Store your screwcap tube in the refrigerator until
the next laboratory period (or proceed to step 2
of Lesson 2).
35
Centrifuge
56°C, 10 min
Sheath
Bulb
Water bath
100°C, 5 min
Water bath
Lesson 1 Hair Follicle DNA Template Preparation