Bio-Rad Ligation and Transformation Module - Manual (Page 8)

When Activity to Complete Duration
At least 1 day prior to Run a PCR reaction in thermal 3–4 h
starting the Ligation cycler to amplify a
and Transformation gene of interest
module Electrophorese the PCR 1 h
products (optional)
Purify PCR products 0.5 h
At least 3 days prior to Prepare LB and LB Amp 0.5 h
the transformation step IPTG agar plates
At least 2 days prior to Prepare LB broth 0.5 h
the transformation step Streak E. coli on a starter LB 5 min
agar plate
Grow E. coli starter plate at 37°C 16+ h
As late as possible the Inoculate starter culture 5 min
day before the Incubate starter culture at
transformation step 37°C in a shaking 8+ h
water bath or incubator
Day of ligation step Ligate PCR product 1 h
Note: Bolded steps use reagents from the Ligation and Transformation
module.
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