Bio-Rad Ligation and Transformation Module - Manual (Page 34)

Experimental Procedure for
Transformation
15. Label one microcentrifuge tube
with your initials and
"transformation."
16. Pipet 5 µl of your ligation reaction
into your transformation
microcentrifuge tube. Keep on
ice.
17. Using a fresh tip, very gently
pipet the competent cells up and
down two times then pipet 50 µl
of competent cells into your
transformation tube. Very gently
pipet up and down two times to
mix and return to ice.
18. Incubate the transformation on
ice for 10 min.
30
Ice
Ice
Ligation
Ligation Competent
cells