Bio-Rad Ligation and Transformation Module - Manual (Page 10)

Kit Inventory Checklist
This section lists equipment and reagents necessary to perform the ligation
and transformation protocol in your classroom or teaching laboratory. Each
kit contains sufficient materials for 12 student workstations, 12 ligation
reactions, and 24 transformations. We recommend that students are
teamed up – two to four students per workstation. Please use the checklist
below to confirm inventory.
Kit Components Number/Kit (
✔)
T4 DNA ligase, 10 µl 1
❒
Ligation reaction buffer (2x concentration), 100 µl 1
❒
Proofreading polymerase, 10 µl 1
❒
pJet1.2 blunted vector, 10 µl 1
❒
Sterile water, 1 ml 1
❒
Bgl II restriction enzyme, 50 µl 1
❒
10x Bgl II reaction buffer, 1 ml 1
❒
Isopropyl
b-D-1-thiogalactopyranoside (IPTG), 1 M, 0.1 ml
1
❒
Transformation reagent A, 1.25 ml 4
❒
Transformation reagent B, 1.25 ml 4
❒
C-growth medium, 30 ml 1
❒
Microcentrifuge tubes, clear, 1.5 ml 30
❒
Microcentrifuge tubes, multicolor, 2.0 ml 120
❒
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