Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 22)

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3.3 General Considerations
3.3.1 Sample
The sample should be clarified using a 0.45 or
0.22 µm filter prior to loading it to the media
plate. Centrifugation prior to filtration is highly
recommended for samples containing a relatively
high concentration of suspended solids.
3.3.2 Media Plate Handling
The media plate should always be placed on
top of a collection plate. Placing the media plate
in direct contact with any surface can result in
leakage of liquid from its wells.
To minimize evaporation during long incubation
times, a self-adhesive microplate seal should be
used to cover the media plate. This seal will also
protect the media plate from contamination.
3.3.3 Liquid Transfer and Buffer Preparation
In cases where a pipetting robotic platform is not
available, multichannel pipets should be used for
liquid dispensing. Using 96-deep well plates to
hold solutions that will be used in the media plate
will save time.
3.3.4 Liquid Removal
The two alternatives for separating the liquid
from the chromatographic media are vacuum
filtration and centrifugation. Vacuum filtration is
done using a 96-well plate vacuum manifold.
Compared to centrifugation, vacuum filtration has
a higher risk of well-to-well cross contamination
during liquid removal due to the rheological
properties of the solution to be vacuumed.