Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 19)

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Csalt (M) …
C
0
(mg/mL) 1 2 3 … 10 11 12
C
1
A
C
2
B
C
3
C
C
4
D
C
5
E
C
6
F
C
7
G
C
8
H (A)
Csalt
1
Csalt
4
C
0
(mg/mL) C
1
C
2
C
3
… C
10
C
11
C
12
Salt (M) 1 2 3 … 10 11 12
A
B
C
D
E
F
G
H
(B)
Csalt
1
Csalt
2
Fig. 3. Schematic representation of the plate
configuration for isotherm experiments using a fixed
phase ratio, and variable initial solute concentration
(C
0
). A, configuration suitable for an 8-channel pipet;
B, configuration suitable for a 12-channel pipet. This
example suggests performing three replicates for each salt
concentration.
A
B