Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 9)

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2.1.3 For BioLogic ™ LP system, 0.8 or 1.6 mm tubing
is recommended. Use fitting from IDEX Health &
Science P-642 female luer to male
10–32 adaptor to connect to column. Use either
0.8 mm barb to male luer (catalog #731-8224),
1.6 mm barb to male luer (catalog #731-8225),
or 3.2 barb to male luer (catalog #731-8226) to
attach to the respectively-sized tubing on the
BioLogic LP system.
2.2 Connecting the Column to a System
2.2.1 Mount the column in a vertical position with the
arrow pointed downward.
2.2.2 Remove the stop plugs from the top and
bottom of the column.
2.2.3 Connect the inlet tubing to the solvent delivery
system and with a flow rate of 0.2 ml/min,
connect the inlet tubing to the column. Push the
tubing in until it bottoms firmly and tighten the
1/16" fingertight male fitting to the female end
piece on the column.
2.2.4 When the solvent is flowing freely from the
outlet end of the column, connect the outlet
tubing to the column by pushing the tubing
in until it bottoms firmly and tighten the
1/16" fingertight male fitting to the female
end piece on the column. Ensure that the
solvent is present in the column assembly,
and then connect it to the detector of the
chromatography system.