Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 12)

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Section 4
Cleaning-in-Place (CIP) and
Sanitation
If a column no longer yields reproducible results, the
media may require thorough CIP and sanitation to remove
strongly bound contaminants. Acceptable CIP agents
include 25% acetic acid, 8 M urea, 1% Triton X-100, 6
M potassium thiocyanate, 70% ethanol, 30% isopropyl
alcohol, 1 N NaOH, and 6 M guanidine hydrochloride.
1. Sanitize the support in the column with 2–4 bed
volumes of 1.0 N NaOH while maintaining a contact
time of at least 40 min.
2. To reequilibrate the column, wash it with 2–4 bed
volumes of 0.5–2 M NaCl solution (containing 50–100
mM buffer).
3. If lipid removal is required, wash the column with a
20–70% ethanol solution.
4.1 Autoclaving
Foresight™ columns are not autoclavable.
4.2 Storage
After washing the columns with deionized water,
Foresight columns should be purged, stored in the
recommended solution for the specific media, and
capped for extended storage.