Bio-Rad Media Sampler Pack - Manual (Page 4)

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Section 4
Technical Specifications and Characteristics
Macro-Prep t-butyl and Macro-Prep methyl media are based on rigid, epoxy-
activated methacrylate beads derivatized with t-butyl or methyl groups,
respectively, and carboxyl groups (Figure 1). These products are shipped as a
slurry of 50% settled media in 20% ethanol. Table 1 lists the properties of the two
media. The rigidity of these media allows high flow rates without bed compression
(Figure 2). Testing in small laboratory-scale columns (10 mm in diameter x 100 mm
in height) showed no bed compression at a pressure of 7 bar and a flow rate of 40
ml/min (3,000 cm/hr). This property of the media provides for high throughput
during loading, column washing, equilibration, and cleaning.
0
5
10
15
20
25
30
35
40
45
37 75 114 159 195 233 271 313 359 409 458 487
Flow rate (cm/hr)
P
re
ssu
re
(p
si
)
0
1
2
3
P
re
ss
ure
(b
ar
)
Fig. 2. Pressure vs. flow for Macro-Prep media. Media bed dimensions were
140 mm diameter by 250 mm length
.
Fig. 1. Macro-Prep HIC: Macro-Prep t-butyl contains t-butyl and carboxyl groups;
Macro-Prep methyl contains methyl and carboxyl groups.
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