Bio-Rad Media Sampler Pack - Manual (Page 11)

9
Sanitization and Long Term Storage
Sanitization is the reduction of bioburden, i.e. microorganisms and spores in the
column. Before long-term storage, Macro-Prep HIC media should be cleaned and
sanitized.
Procedure:
Cleaning: After elution, clean the column with 8 CV of 1% acetic acid in 0.12 M
phosphoric acid, pH 1.5, <100 cm/hr. If not satisfactorily cleaned, continue to
wash with 5 CV of 6 M guanidine-HCl, <100 cm/hr. If still not clean, wash the
column with 5 CV of 20% ethanol, <100 cm/hr.
To sanitize and store: Wash the column with 5 CV of 1% acetic acid in 0.12 M
phosphoric acid, pH 1.5. Store the column at 4–40°C.
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