Bio-Rad Bio-Rex® 70 Cation Exchange Resins - Manual (Page 9)

Table 4. (Continued)
Purification of inhibitory pro- Fearon, D. T., Proc. Nat. Acad. Sci. USA, 76,
tein of human erythrocytes 5867 (1979).
Measurement of glyco- Trivelli, L. A., Ranney, H. M. and Lai, H. T.,
sylated hemoglobin N. E. J. Med., 284, 353 (1971); Schifreen, R.
S., Hickingbotham, J. M. and Bowers, G. N.
Jr., Clin. Chem., 26, 466 (1980).
Purification of protein aller- King, T. P., Sobotka, A. K., Alagon, A.,
gens of white-faced yellow Kochoumiam, L. and Lichtenstein, L. M.,
hornet, and yellow jacket Biochemistry, 17, 5165 (1978).
venoms
Purification of oximdoleala- Shrake, A. and Rupley, J. A., Biochemistry,
nine -62 lysozyme 19, 4044 (1980).
Isolation of gentamicin Habbal, Z. M., Clin. Chim. Acta, 95, 301
from serum (1979).
Isolation of brain histamine Lewis, S. J., Fennessy, M. R., Laska, F. J.
and Taylor, D. A., Agents and Actions, 10,
197 (1980).
Determination of thiamine Ellefson, W. C., Richter, E., Adams, M. and
in food Baillies, N. T., J.A.O.A.C., 64, 1336 (1981).
Purification of toxins Levinson, S. R., Curatolo, C. J., Reed, J. and
Raftery, M. A., Anal. Biochem., 99, 72 (1979).
Isolation of siderophores Frederick, C. B., Szaniszlo, P. J., Vickrey,
from fungus P. E., Bentley, M. D. and Shive, W.,
Biochemistry, 20, 2432 (1981).
Purification of E. coli photo- Koka, P., Biochemistry, 20, 2914 (1984).
reactivity enzyme
Purification of polypeptides Hamilton, S. L., Yatani, A. and Hawkes,
from croatalus atrox venom M. J., et al., Science, 229, 182 (1985).
Purification of a yeast Johnson, A. W. and Demple, B., J. Biol.
DNA repair enzyme Chem., 263, 18009 (1988).
13
7.1 Protein, Peptide, and Amino Acid
Separations
Proteins may be separated from one another using
the macroporous Bio-Rex 70 cation exchange resin,
because this resin has pores of sufficient size to admit the
proteins. The effective exclusion limit of this resin has
not been established, however, from the many
separations reported, the limit exceeds 75,000 daltons.
Table 4. Applications of Bio-Rex 70 Resin
Purification of the rep pro- Scott, J. F. and Kornberg, A., J. Biol. Chem.,
tein of E. coli 253, 3292 (1978).
Purification of human saliva Vasstrand, E. N. and Jensen, H. B., Scand. J.
lysozyme Dent. Res., 88, 219 (1980).
Purification of neurotoxins Karlsson, E., Eaker, D., Fryklund, L. and
from sea snake venom Kadin, S., Biochemistry, 11, 4628 (1972).
Purification of initiation Stringer, E. A., Chaudhuri, A. and Maitra,
factor 2 from calf liver U., J. Biol. Chem., 254, 6845 (1979).
Purification of histones D’Anna, J. A., Strniste, G. F. and Gurley, L.
R., Biochemistry, 18, 943 (1979); Thompson,
J. A., Stein, J. L., Kleinsmith, L. J. and Stein,
G. S., Science, 194, 428 (1976).
Purification of restriction Green, P. J., et al., Nucleic Acids Research,
endonucleases 5, 2373 (1978).
Purification of human Savage, C. R. Jr. and Harper, R., Anal.
epidermal growth factor Biochem., 111, 195 (1981).
Purification of human C1q Tenner, A. J., Lasavre, P H. and Cooper, N.
R., J. Immunol., 127, 648 (1981).
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