Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 6)

4
Fig. 1. A simulated chromatography profile from which HETP and As values are
calculated.
a b 10% of peak
h i h
W
h,
width at half heig
Bed height = 20 cm
Column Volume = 20 L
Flow rate = 100 cm/h
Ve = 10 L
W
h
= 1 L
HETP = 0.036 cm
a = 1
b = 1.2
A
s
= 1.2
Bed height = 20 cm
Column volume = 20 L
Flow rate = 100 cm/h
V
e
= 10 L
W
½h
= 1 L
HETP = 0.036 cm
a = 1
b = 1.2
A
s
= 1.2
W
½h
width at half height
10% of peak a b
10010339A.qxp 3/3/2008 7:50 AM Page 5