Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 3)

1
Section 1
Introduction
UNOsphere rapid S ion exchange media is a hydrophilic spherical polymeric bead
designed for the separation of proteins, nucleic acids, viruses, plasmids, and other
macromolecules. The UNOsphere beads are designed for high capacity, low
backpressure, and high productivity.
Section 2
Technical Description
Table 1. Characteristics of UNOsphere Media.
UNOsphere rapid S
Type of ion exchanger Strong cation
Functional group –SO3 –
Total ionic capacity 140 µeq/ml
Dynamic binding capacity*
150 cm/hr 60 mg/ml
600 cm/hr 30 mg/ml
Shipping counterion Na+
Median particle size 100 µm
Recommended linear flow rate range 50–800 cm/hr
Chemical stability
1.0 M NaOH (20°C) £800 hr
1.0 M HCl (20°C) £200 hr
Gel bed compression ratio 17–18%
pH stability 1–14 short term
2–13 long term
Autoclavability (121ºC, 30 min) Yes
Antimicrobial agent 20% ethanol
Regeneration 70% ethanol or 1–2 M NaCl
Storage conditions 20% ethanol or 0.1 M NaOH
*10% breakthrough capacity determined with 4.5 mg/ml human IgG.
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