Bio-Rad Media Sampler Pack - Manual (Page 3)

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Section 1
Introduction
UNOsphere Q and S ion exchange media are hydrophilic spherical polymeric beads
designed for the separation of proteins, nucleic acids, viruses, plasmids, and other
macromolecules. The UNOsphere beads are designed for high capacity, low
backpressure, and high productivity.
Section 2
Technical Description
Table 1. Characteristics of UNOsphere Media.
UNOsphere Q UNOsphere S
Type of ion exchanger Strong anion Strong cation
Functional group –N+(CH3)3 –SO3 –
Total ionic capacity 120 µeq/ml 260 µeq/ml
Dynamic binding capacity*
150 cm/hr 180 mg/ml 60 mg/ml
600 cm/hr 125 mg/ml 30 mg/ml
Shipping counterion Cl– Na+
Median particle size 120 µm 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr 50–1,200 cm/hr
Chemical stability
1.0 M NaOH (20°C) ≤2,000 hr ≤2,000 hr
1.0 M HCl (20°C) ≤200 hr ≤200 hr
Volume changes
pH 4–10 <5% <5%
0.01–1.0 M NaCl <5% <5%
Gel bed compression ratio*** 25% ± 3% 25% ± 3%
pH stability 1–14 1–14
Autoclavability (121ºC, 30 min) Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol
Regeneration 70% ethanol or 1–2 M NaCl 70% ethanol or 1–2 M NaCl
Storage conditions 20% ethanol or 0.1 M NaOH 20% ethanol or 0.1 M NaOH
*10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA
(UNOsphere Q) in a 1.1 x 20 cm column.
**UNOsphere media packed to a 20 cm bed height and run at 1,200 cm/hr generate less than 2 bar
backpressure.
***A compression ratio is for axially compressed columns packed at a constant flow rate (100–1,200 cm/hr) or
constant pressure of 1–2 bar. Gel bed will rebound to its original volume 15–20 min after the pump stops.
Columns with small ID may not rebound to original volume due to Wall effects.
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