Bio-Rad Affi-Gel 15 Gel - Manual (Page 2)

Table of Contents
Section 1 Introduction...................................... 1
Section 2 Coupling Chemistry......................... 2
Section 3 General Coupling Conditions ......... 5
3.1 pH Dependence ......................................... 5
3.2 Temperature .............................................. 12
3.3 Time .......................................................... 12
3.4 Ligand Concentration ................................ 12
Section 4 Recommended Storage Conditions 16
Section 5 General Instructions ........................ 16
5.1 Aqueous Coupling..................................... 16
5.2 Anhydrous Coupling ................................. 19
Section 6 Monitoring For Protein Coupling .. 22
Section 7 Troubleshooting ............................... 22
Section 8 Using the Coupled Support ............. 25
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LIT156B 6/17/98 10:40 AM Page i