Bio-Rad DEAE Affi-Gel Blue Gel - Manual (Page 2)

Introduction
DEAE Affi-Gel Blue gel is a bifunctional affinity/ion
exchange chromatography matrix prepared by coupling
Cibacron
® blue F3GA and diethylaminoethyl groups to
Bio-Gel ® A-5m, agarose gel. The Cibacron blue F3GA
functions as an ionic, hydrophobic, or sterically active
binding site for proteins with dinucleotide folds, such as
albumin. The diethylaminoethyl functional group
functions as an anion exchanger and will bind proteins
with isoelectric points higher than the pH of the mobile
phase. This bifunctionality makes DEAE Affi-Gel Blue
gel a very powerful tool. It is possible to obtain highly
purified IgG from serum from a wide variety of species
through carefully optimizing the ionic strength and pH of
the application buffer.
Chromatography on DEAE Affi-Gel Blue gel
provides a convenient initial step in the purification of
serum proteins. After the IgG has eluted, additional
fractions can be obtained from the DEAE Affi-Gel Blue
gel by elution with an ionic strength gradient. DEAE
Affi-Gel Blue gel has a greater binding affinity for serum
albumin than DEAE ion exchangers, and offers a
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