Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges - Manual (Page 5)

stranded DNA. Detailed product information is given
in Tables 1 and 2.
Table 1: Bio-Scale Mini CHT Ceramic
Hydroxyapatite Cartridge Specifications
Size 5 ml bed volumes
Dimension 40 mm length x 12.6 mm inner
diameter
Maximum pressure tolerance 45 psi
Recommended flow rate 5–10 ml/min (140–481 cm/hr)
Maximum flow rate 20 ml/min (963 cm/hr)
Fittings Female luer fitting inlet and
male luer fitting outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping condition Dry
Storage recommendation 0.1 N NaOH
Autoclavability Not autoclavable
3
The Bio-Scale Mini CHT cartridges are packed with
Type I or Type II ceramic hydroxyapatite supports.
These supports are based on hydroxyapatite, a
form of calcium phosphate used in chromatographic
separations of biomolecules. CHT ceramic
hydroxyapatite is a spherical, macroporous form of
hydroxyapatite. Unlike most other chromotagraphic
absorbents, CHT is both the ligand and the support
matrix. CHT Type I has a higher protein binding
capacity and greater capacity for acidic proteins.
CHT Type II has a lower protein binding capacity
but provides better resolution of nucleic acids and
certain other proteins. The Type II material also has a
very low affinity for albumin and is especially suitable
for the purification of many species types and classes
of immunoglobulins. Applications of hydroxyapatite
chromatography include the purification of different
subclasses of monoclonal and polyclonal antibodies,
antibodies that differ in light chain composition,
antibody fragments, isozymes, supercoiled DNA from
linear duplexes, and single-stranded from double
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