Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges - Manual (Page 13)

Section 5
Care of the Cartridge
5.1 Cleaning
After repeated use, a CHT cartridge may require
thorough cleaning and regeneration to remove
bound contaminants. Most bound contaminants
may be removed by the following protocol:
Step 1. Wash the cartridge with 3–5 column
volume (5 ml/min for the 5 ml) of 500 mM
potassium or sodium phosphate, then
5 column volumes of starting buffer.
Step 2. Equilibrate the cartridge with at least
5 column volumes (5 ml/min for the 5 ml)
of starting buffer. If bound contaminants
persist after following the steps above, use
one of the alternative procedures listed in
wash alternatives.
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C CH HT T S Su uiittaab biilliittyy
p pH H B Bu uffffeerr 1 10 0 ccyycclleess 5 50 0 ccyycclleess
7.0 MES + 5 mM PO4 + n/a
7.0 Acetate + 5 mM PO4 + n/a
7.0 Imidazole + 5 mM PO4 + +
7.0 Glycine + 5 mM PO4 + n/a
7.0 Arginine + 5 mM PO4 + n/a
7.0 HEPES + 5 mM PO4 + n/a
7.0 Tris + 5 mM PO4 + n/a
7.5 Phosphate (5 mM) + n/a
7.5 MES + 5 mM PO4 + n/a
7.5 Imidazole + 5 mM PO4 + +
7.5 Acetate + 5 mM PO4 + n/a
7.5 HEPES + 2 mM PO4 + n/a
7.5 HEPES + 5 mM PO4 + n/a
7.5 Tris + 2 mM PO4 + n/a
7.5 Tris + 5 mM PO4 + +
8.5 Tris + 5 mM PO4 + n/a
* All experiments performed in small scale columns. Each cycle used approximately 35 column
volumes of buffer to simulate an equilibration and long gradient, as well as five column volumes
of 1 N NaOH to simulate regeneration.
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