Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 10)

Table 2. Technical Description of UNOsphere
SUPrA Affinity Chromatography Media.
Composition Highly crosslinked polyacrylamide polymer
Particle size range 53–61 µm
Ligand Recombinant protein A
Coupling chemistry Epoxy
Dynamic binding capacity* 150 cm/hr 30 ± 3 mg/ml
300 cm/hr 25 ± 2 mg/ml
450 cm/hr 20 ± 2 mg/ml
Chemical stability** 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Working pH range 3–11
Cleaning-in-place (CIP) 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase 100–600 cm/hr
velocity range
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C
* Minimum 20 mg/ml at 300 cm/hr; 10% breakthrough capacity determined
with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance after 24-hour storage
at room temperature.
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