Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 7)

Fig. 5.1. Protein desalting with the Econo-Pac
10 DG desalting column.
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5. Allow entire sample to enter the column. Add (3.0
ml - sample volume) of buffer. Allow buffer to enter
the column and discard the effluent.
6a. Add 1.5 x sample volume or 4ml of buffer
(whichever is less) to elute the higher molecular
weight component(s). Collect this fraction from the
column. For a more precise collection method refer
to step 6b.
6b. Elute the desired components with 8 ml of buffer,
while collecting 1 ml fractions from the column.
Analyze the fractions with the appropriate detector
and plot the results against the fraction number. Pool
the desired fractions (See Figure 5.1).
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Conditions
Column:
Econo-Pac 10 DG desalting column
Sample: BSA in 250 mM NaCI
Buffer: H
2
O
Flow rate: 1.0 ml/min
Peaks:
1. BSA
2. NaCI
Conductivity
O.D. 280
1
2
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