Bio-Rad Bio-Scale™ MT High-Resolution Empty Columns - Manual (Page 9)

Bio-Scale MT Column Instruction Manual 5
9. Gently turn the lock nut until the upper bed support is in the
desired position.
10. Attach upper (1) and lower (11) end fittings.
2.3 Column Packing
Always follow the manufacturer’s instructions for packing a
particular type of media. Generally, slurry packing will give better
chromatographic performance than dry packing. The following
guidelines may be useful:
Dry packing
Particle size Ž 20 µm
Slurry packing Particle size < 20 µm
Dry Packing
Assemble the column according to the following figure.
Fig. 2 Column tube assembly.
1. Holding the column tube vertically, fill one half with the packing
media.
2. Tap the side of the column until the media settles.
3. Fill the column with the remainder of the media and repeat
step 2.
4. When the column is filled to the desired level, complete the
column assembly as described in Section 2.2, making sure the
flow direction arrow on the glass tube is pointing in the current
direction.
FLOW