Bio-Rad Bio-Scale™ MT High-Resolution Empty Columns - Manual (Page 11)

Bio-Scale MT Column Instruction Manual 7
8. Pass 5 bed volumes of buffer through the column at a flow
rate 20% higher than the operating flow rate.
9. If necessary, stop the flow and lower the upper bed support
via the lock nut to remove any head space that might have
formed between the frit and the top of the bed.
10. Equilibrate the column at the desired flow rate.
2.4 Connections to Chromatography Systems
Bio-Scale MT columns can be used with any chromatography
system that can provide the appropriate flow rate and pressure.
Each column is supplied with 1/4-28 fittings for connection to
Bio-Rad’s BioLogic System, M6 fittings for connection to FPLC®
systems, and 10-32 fittings for connection to HPLC systems. Inlet
and outlet tubing must be 1/16” OD. Inner diameters of 0.020” or
0.030” in Tefzel®, PTFE or PEEK material are recommended.