Bio-Rad BioLogic DuoFlow Valves - Manual (Page 3)

Example 2. Buffer Selection
Placed upstream of the BioLogic Gradient Pumps, the V9-8 or AV9-8 allows selection of up to 8 different
solutions. When used in this manner, buffers for different types of chromatography and for column sanitation
may be used as part of an automated separation procedure. For an ion exchange separation, often several buffer
systems with different pH conditions are used to optimize a separation. By using the 8-ports, eight different
buffering systems are set up for evaluation. An example of this configuration is shown below. For this
example, 1/8" OD / 0.062" ID PTFE tubing is recommended.
NOTE
: During valve position changes, either stop the Gradient Pump or run the Gradient Pumps at a slow flow rate (<1
ml/min). Failure to do so could lead to generation of air bubbles in the Gradient Pumps.
1
2
3
4
5
6
7
COLUMN
V7-3
OR
AV7-3
A
GRADIENT
PUMP
INJECT
VALVE
B
1
2
3
4
5
6
9
7
8
A1
A2
A3
A4
B1
B2
B3
B4
1
2
3
4
5
6
9
7
8
V9-8 OR AV9-8 V9-8 OR AV9-8