Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 26)

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Possible Causes
High Inter-Assay CV
Standards were not reconstituted
consistently between assays
Reconstituted standards and
diluted samples were not stored
properly
Bottom of filter plate not dry
Possible Solutions
Incubate the reconstituted standards
for for the recommended time
period. Always be consistent with the
incubation time and temperature.
Diluted samples should be prepared
on ice as instructed. Prior to
plating, the reconstituted standards
and diluted samples should be
equilibrated to room temperature.
Dry the bottom of the filter plate with
absorbent paper towel (preferably
lint-free) to prevent cross-well
contamination.
Troubleshooting Guide
This troubleshooting guide addresses problems that may be encountered
with Bio-Plex Pro
™ RBM assays. If you experience any of the problems
listed below, review the possible causes and solutions provided. Poor
assay performance may also be due to the Bio-Plex
® suspension array
reader. To eliminate this possibility, use the validation kit to validate all the
key functions of the array reader and to assist in determining whether or
not the array reader is functioning properly.