Bio-Rad Human Metabolic and Hormone Assays - Manual (Page 20)

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7. Read Plate
Bio-Plex Manager software is recommended for all Bio-Plex Pro assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions on using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager Software
Version 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes used in the reading, the plate wells
to be read, sample information, the values of standards and controls,
and instrument settings. Protocols may be obtained from within Bio-Plex
Manager software version 6.1 or created from the File menu.
To create a new protocol, select File, then New from the main menu.
Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay (optional).
2. Click Select Analytes and create a new panel.
a. Click Add Panel in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the assay
dropdown menu. If using Bio-Plex Manager version 5.0 or lower, select
MagPlex from the assay dropdown menu.
b. Click Add. Enter the bead region number and name for the first
analyte. Click Add Continue to repeat for each analyte in the assay.
Refer to the bead regions in parentheses ( ) listed on the peel-off label
provided with the standards.
For reference, bead regions for the individual assays are listed in Table 8.
c. Click Add when the last analyte has been added and click OK to save
the new panel.