Bio-Rad Ethidium Bromide Solution - Manual (Page 5)

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2.2 CsC1 Gradient Instructions
Use 10 mg/ml ethidium bromide solution as
provided. Add 0.8 ml of ethidium bromide
solution for every 10 ml of cesium chloride/DNA
solution to yield a final ethidium bromide
concentration of 740 µg/ml.
1
1. Maniatus, T., Fritsch, E.F. and Sambrook, J. Molecular
Cloning: A Laboratory Manual, Cold Spring Harbor
Laboratory (1989).
Section 3
Health Hazards
Ethidium bromide is a toxic chemical and a
mutagen. It causes eye and skin irritation. Wear
gloves at all times and wash contaminated skin
with water. Clean up liquid spills by absorbing
the liquid with absorbent material. Spray the area
with a 10% bleach solution, clean up with
absorbent material, rinse with water, and dry the
area with paper towels. Refer to the Risk and
Safety Phrases and the MSDS in this manual for
additional information.
3.1 Risk and Safety Phrases
Hazardous products are marked with
Hazard Symbols and Risk and Safety Phrases in
accordance with the rules of the European
Community Commission. Refer to the catalog
for a complete listing of Hazard Symbols and
Risk and Safety Phrases.
R 46
S 44, 53
EINECS 2149846
RTECS SF7950000
CASNr 1239-45-8
For laboratory use only.
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