Bio-Rad Ethidium Bromide Solution - Manual (Page 4)

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Wear gloves at all times and wash contaminated
skin with water. Refer to Section 3 of this manual
for additional Health Hazard information.
2.1 Gel Staining Instructions
Make a 0.5 µg/ml working solution accord-
ing to the volume required:
0.5 µg/ml (1 liter) 0.5 µg/ml (200 ml)
Add one drop (50 µl) Add 10 µl of 10 mg/ml
of 10 mg/ml ethidium ethidium bromide solu-
bromide solution to tion to 200 ml ddi water
1000 ml ddi water
Soak the gel for 15 minutes with gentle
agitation. Longer staining times will result in
high background. Rinse the gel with ddi water
and destain with fresh ddi water for 15 minutes
with gentle agitation. At this point, the gel
should be ready for photographing, although
another 15 minute rinse with fresh ddi water will
reduce background further.
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1.1 Specifications
Contents Ethidium bromide solution,
10 mg/ml, 10 ml
Application Agarose and polyacrylamide gel
staining
Large scale plasmid preparation
using cesium chloride gradients
Storage May be stored indefinitely in a
lightproof container at room tem-
perature
Section 2
Instructions for Use
Ethidium bromide is used to visualize DNA
in agarose gels and in many cesium chloride gra-
dient protocols. The 10 mg/ml ethidium bromide
stock solution can be diluted for gel staining or
used as provided for CsC1 gradient protocols.
Caution: Ethidium bromide is a toxic chemical
and a mutagen. It causes eye and skin irritation.
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