Bio-Rad Model 785 Vacuum Blotter - Manual (Page 3)

Table of Contents
Section 1 General Information ................................................................................................1
1.1 Safety/Definition of Symbols ....................................................................................1
1.2 Introduction to Model 785 Vacuum Blotter................................................................1
1.3 List of Parts ................................................................................................................1
1.4 Features ......................................................................................................................1
1.5 Specifications..............................................................................................................2
Section 2 Description of Major Parts ......................................................................................3
2.1 Base Unit ....................................................................................................................3
2.2 Vacuum Stage ............................................................................................................3
2.3 Porous Vacuum Plate ..................................................................................................3
2.4 Window Gasket ..........................................................................................................3
2.5 Sealing Frame ............................................................................................................4
2.6 Vacuum Regulator ......................................................................................................4
2.7 Vacuum Pump ............................................................................................................4
2.8 Lid ..............................................................................................................................4
Section 3 Operating Instructions ............................................................................................5
3.1 Vacuum Blotter Setup ................................................................................................5
3.2 Preparation for Vacuum Transfer................................................................................5
3.3 Gel Pretreatment ........................................................................................................5
3.4 Vacuum Transfer ........................................................................................................7
3.5 Post Transfer ..............................................................................................................8
Section 4 Cleaning and Maintenance ......................................................................................8
4.1 Cleaning......................................................................................................................8
4.2 Replacement Parts ......................................................................................................8
Section 5 Troubleshooting Guide ............................................................................................9
5.1 Operational Troubleshooting ......................................................................................9
5.2 Southern Hybridization Troubleshooting ................................................................10
5.3 Repairing Cracked Gels ............................................................................................11
Section 6 Equipment and Accessories ..................................................................................12
6.1 Model 785 Vacuum Blotter and Accessories............................................................12
6.2 Electrophoresis Reagents..........................................................................................12
6.3 Blotting Reagents......................................................................................................13
Section 7 Appendix..................................................................................................................13
7.1 Facts About Vacuum Transfer ..................................................................................13
7.2 Required Solutions and Equipment ..........................................................................15