Bio-Rad Sub-Cell® Model 192 Cell - Manual (Page 3)

Table of Contents
Page
Warranty Information ..........................................................................Inside Front Cover
Section 1 General Information....................................................................................1
1.1 Introduction ................................................................................................................1
1.2 Safety ..........................................................................................................................1
1.3
List of System Parts....................................................................................................3
1.4 Specifications .............................................................................................................5
Section 2 Operating Instructions ................................................................................6
2.1 DNA Gel Preparation.................................................................................................6
2.2 Comb Set-up...............................................................................................................7
2.3 Casting Agarose Gels .................................................................................................8
2.4 Recirculation Ports ...................................................................................................11
2.5 Electrophoresis .........................................................................................................12
2.6
Nucleic Acid Staining and Visualization.................................................................13
2.7 Note on Blotting .......................................................................................................14
Section 3 Gel and Electrophoresis Reagents Preparation......................................14
3.1 Electrophoresis Buffer Preparation..........................................................................14
3.2 DNA and RNA Gel Preparation ..............................................................................15
3.3 RNA Sample Preparation.........................................................................................15
3.4 DNA and RNA Sample Loading Dye .....................................................................16
3.5 Gel Staining Solution ...............................................................................................16
Section 4 Care and Maintenance ..............................................................................16
4.1 Cleaning Sub-Cell System Components..................................................................16
4.2 Compatible Cleaning Agents ...................................................................................16
4.3 Maintenance Schedule .............................................................................................17
4.4 Electrode Replacement.............................................................................................17
4.5 RNase Decontamination ..........................................................................................18
Section 5 Troubleshooting..........................................................................................19
Section 6 Ordering Information ...............................................................................20
6.1 Sub-Cell Model 96 and 192 Systems.......................................................................20
6.2 Sub-Cell Model 96 and 192 System accessories.....................................................20
6.3 Related Bio-Rad Products........................................................................................21
Section 7 References ...................................................................................................25
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