Bio-Rad ReadySub-Cell GT Cells - Manual (Page 18)

Symptoms Cause Solutions
Sample wells cast Comb should be placed 1 to 2 mm
through the gel. Sample above the base of the running surface.
leaks along bottom of
running surface.
Sample overload Dilute sample.
Bands sharp but too Gel agarose percentage too high Lower agarose percentage.
few bands seen
Incomplete digestion Check enzyme activity, digest further.
High MW bands Gel agarose percentage too low Increase agarose percentage.
sharp; Low MW Switch to polyacrylamide.
bands smeared
Gels crack Too high voltage gradient, Reduce voltage. Run gel at lower
especially with low melting temperature.
temperature agarose or
low gel strength gels
Section 6
Product Information
6.1 Sub-Cell ® GT Systems
Catalog
Number
Product Description
170-4401 Sub-Cell GT System, with 15 x 10 cm tray
170-4402 Sub-Cell GT System, with 15 x 15 cm tray
170-4403 Sub-Cell GT System, with 15 x 20 cm tray
170-4404 Sub-Cell GT System, with 15 x 25 cm tray
170-4481 Sub-Cell GT System, with 15 x 10 cm tray and gel caster
170-4482 Sub-Cell GT System, with 15 x 15 cm tray and gel caster
170-4483 Sub-Cell GT System, with 15 x 20 cm tray and gel caster
170-4484 Sub-Cell GT System, with 15 x 25 cm tray and gel caster
170-4405 Wide Mini-Sub Cell GT System, with 15 x 7 cm tray
170-4485 Wide Mini-Sub Cell GT System, with 15 x 7 cm tray and mini-gel caster
170-4406 Mini-Sub Cell GT System, with 7 x 7 cm tray
170-4486 Mini-Sub Cell GT System, with 7 x 7 cm tray and mini-gel caster
Sub-Cell Systems with PowerPac ™ Basic Power Supply, 100—120/220—240 V
164-0302 Sub-Cell GT Cell and PowerPac Basic Power Supply
164-0301 Wide Mini-Sub Cell GT Cell and PowerPac Basic Power Supply
164-0300 Mini-Sub Cell GT Cell and PowerPac Basic Power Supply
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