Bio-Rad ReadySub-Cell GT Cells - Manual (Page 15)

DNA and RNA sample loading dye 1-2
A convenient 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and 0.25% xylene
cyanole FF in 1x TAE buffer. Only 1—10 ml of the 10x loading dye should be prepared.
RNA sample preparation 1-2
Prior to loading RNA onto an agarose formaldehyde gel prepare each RNA sample as follows:
6 µl RNA in DEPC-treated water
10 µl 5x MOPS buffer (final concentration 1.67x)
9 µl 12.3 M formaldehyde (final concentration 3.7 M)
25 µl formamide (final concentration 50% v/v)
Caution: Formamide is a teratogen. Always wear gloves, safety glasses, and a laboratory coat. Use caution when
handling formamide. Consult the formamide MSDS for more information.
Ethidium bromide solution
Add 10 mg of EtBr to 1 ml distilled water. Bio-Rad offers EtBr solutions (10 mg/ml).
Section 4
Care and Maintenance
4.1 Cleaning Sub-Cell GT Components
1. All Sub-Cell GT system parts should be washed with a mild detergent solution in warm water.
Note: Be careful not to snag or break the electrode wire in the GT base while cleaning.
2. Rinse all parts thoroughly with warm water or distilled water and air dry, if possible.
4.2 Compatible Cleaning Agents
Chemically compatible cleaners must be used to insure long life of parts. These include:
• Aqueous solutions of soaps and mild detergents:
Bio-Rad Cleaning Concentrate (catalog number 161-0722)
Dishwashing liquid
• Organic solvents:
Hexane
Aliphatic hydrocarbons
Do not leave plastic parts to soak in detergents more than 30 minutes. A short detergent rinse typically is all
that is required.
Caution: Do not use the following chemicals to clean Sub-Cell GT parts. Exposure to these chemicals may
cause the plastic parts to crack, craze, etch, or warp.
• Chlorinated hydrocarbons
Carbon tetrachloride
Chloroform
• Aromatic hydrocarbons
Benzene
Phenol
Toluene
Methyl ethyl ketone
Acetone
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