Bio-Rad Model 491 Prep Cell and Mini Prep Cell User Manual

Page 31

Section 5

Disassembling and Cleaning

1. Turn off the power supply, disconnect the cables from the power supply, and re move the lid. Turn off

both the elution and cooling pumps.

2. Disconnect the elution buffer tubing from the cooling core. Close the valve of the cooling buffer outlet on

the lower reservoir and disconnect the cooling pump tubing from the lower reservoir and the cooling
core.

3. Remove the upper reservoir with the gel tube assembly from the lower buffer chamber and pour off the

remaining buffer from both the elution and upper elec trophoresis buffer reservoirs. Disconnect the elution
buffer feedline and cooling buffer feedline from the elution chamber cap. Disconnect the cooling buffer
line from the cooling core and unscrew the black ring nut. Remove the upper reservoir from the gel tube
assembly.

4. Loosen the screws in the elution chamber cap and remove the gel tube assembly from the elution

chamber base. Rinse the support frits and the elu tion frit with deionized water and place them in a
container of buffer. Inspect the dialysis mem brane for tears. If the dialysis membrane is intact, rinse it in
deionized water and place it in a container with buffer.

5. Unlock and turn the cooling core several times to loosen it from the gel. Holding the gel tube in one

hand and the neck of the cooling core in the other, carefully pull out the cooling core. The gel may be
pulled up along with the cooling core. Use a soft implement to remove the gel from the gel tube. Wash
the gel tube and the cooling core with Bio-Rad Cleaning Concentrate (catalog number 161-0722). A
bottle brush may be used to remove residual polyacrylamide from the gel tube and cooling core. Do not
scratch or other wise abrade the interior surface of the glass tube or the glazing of the cooling core.
Insure that the collar of the gel tube assembly is free of residual polyacry lamide.

6. Decant the buffer from the lower reservoir. Rinse the Model 491 prep cell com ponents in deionized

water and dry them in air.