Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 16)

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2. Decant the stacking gel overlay, rinse the surface of the stacking gel with water, and loosen the four
screws holding the column to the casting stand. Carefully re move the gel tube assembly from the
casting stand. Insert a spat ula into the gel re lease slot and use it to gently pry the gel tube assembly off
the cast ing stand. Inspect the lower surface of the gel to make sure it is smooth. Trapped air bubbles
may cause a pitted gel surface which will result in un even elution of proteins from the gel. If a pitted or
otherwise uneven gel surface is observed, pour a new gel.
3. Place the gel tube assembly containing the gel on the elution chamber base. Align the four screws with
the holes in the elution chamber base and hand tighten them.
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