Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 15)

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3.2 Preparing the Frits and Dialysis Membrane
Soak the elution manifold support frit, elution frit, and dialysis membrane in buffer. The frits must be com-
pletely wetted prior to use. To ensure removal of en trapped air in the pores of the frits, place the container
in which the frits are soaking in a vacuum chamber for approximately 10 minutes. Alternatively, the frits can
be soaked in buffer overnight to completely wet them. To maintain the wetting of the frits, store them in buf-
fer.
The dialysis membrane provided with the Model 491 prep cell has a molecular weight cut off of 6,000 daltons.
Dialysis membranes with other pore sizes may be sub stituted for those pro vided. The dialysis membrane
must be soaked in buffer before use and stored in buffer or water between uses. If the membrane be comes
dry between runs, discard it. A properly stored dialysis membrane can be used at least for 5-6 runs. Prior
to each run, inspect the membrane care fully. Discard it if any holes or tears are detected.
3.3 Assembly of the Elution Chamber
1. Insert the soaked support frit into the base of the elution chamber. The stepped support frit is the
thicker of the two frits. Press the frit all the way into the base to form a flat surface on which to place
the dialysis membrane. Place the dialysis membrane on the support frit and the elution frit on the
dialysis membrane. Press the large elution O-ring in the groove around the perime ter of the base.
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