Bio-Rad Model 491 Prep Cell and Mini Prep Cell - Manual (Page 19)

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3.5 Purge the Elution Chamber of Air Bubbles
The elution chamber must be purged of air prior to running the cell. To do this, at tach a 50 ml syringe to the
elution buffer outlet tubing at the top of the cooling core. Gently pull elution buffer through the elution
chamber into the 50 ml syringe. To remove air bubbles trapped in the channels of the gray elu tion gasket,
gently push buffer (not air) back into the elution chamber with a full syringe. As you do this, you will see the
air bubbles trapped in the elution chamber exit through the port into the elution buffer reservoir. It may help
to lean the prep cell so that the feed line port on the elution chamber is up permost on the chamber making it
easier for bubbles to flow up and out into the elution buffer reservoir.
Release the syringe and attach the elution buffer out let tubing to a peristaltic pump. Set the elu tion pump
speed to 1 ml/min. Connect the outlet of the pump to a frac tion collector.
Fig. 8. Elution buffer pathway. Elution buffer is pulled from the elution buffer reservoir to the elution buffer port on the elution chamber
cap. The flow is directed through a series of channels in the gray elution chamber gasket to the perimeter of the elution frit. From there,
elution buffer and sep arated proteins emerging from the gel are drawn radially to the center of the elution frit and out through the elution
tube in the center of the cooling core. The elution tube is connected to a peristaltic pump, which drives the elution buffer to a detector
(optional) and a fraction collector.
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