Bio-Rad Model 583 Gel Drying Supports - Manual (Page 9)

6
Set Up and Operation
Table 3 (continued)
Gel Dryer Operation
Step Procedure
7. Start the drying cycle.
8. Adjust the temperature or time at
any time during the drying cycle.
9. When the cycle is complete and the
gel is dry, break the seal on the
Sealing Gasket before turning off
the vacuum pump.
10. Turn off the vacuum pump.
11. To repeat the current cycle and
settings, simply press the
START/STOP key.
2.3 DETERMINING IF YOUR GEL IS DRY
Carefully note the appearance of the gel at the start of drying. The initial hydrated thickness will cause the
transparent Sealing Gasket to assume the shape of the gel once the vacuum seal is formed. During drying,
the gel will compress as the liquid is removed. When the gel is completely dry, the gasket will be perfectly
flat for gels < 10% T, but retain a slight gel shape for gels > 10% T. Gradient gels, depending on the percent
range, will be flat at one end and show some gel shape at the other. Feel the gel through the gasket. A dry
gel will feel warm to the touch across its entire surface. Incompletely dried gels will feel cool in some areas
and warm in other areas.
Description
a. Press the START/STOP key.
b. During dryer operation, the indicator light above
the
START/STOP key is lit.
Press the TEMP or TIME key to view the actual tem-
perature or time remaining. To make an adjustment,
a. Press the TEMP or TIME key twice.
b. Press the RAISE or LOWER key to change the
value.
This prevents the liquid in the vacuum line from flow-
ing back to the gel dryer.
Do not continue running the vacuum pump after the
vacuum seal is broken.