Bio-Rad Mini-PROTEAN II Multiscreen Apparatus - Manual (Page 6)

Fig. 1. Assembly of the multiscreen apparatus.
3. Lay the blocked blot on the gasket with the antigen side facing up. Center the membrane
so that the channels of the sample template cover the length of the blotted sample.
4. Place the sample template on top of the membrane. The guide pins insure that the template
will be properly aligned. Finger tighten the four screws. When tightening the screws, use
a diagonal crossing pattern to insure even pressure on the membrane surface (see Figure
2). The multiscreen apparatus is ready for sample application.
Note: Use of excessive force when tightening the screws is not necessary to prevent well-
to-well leakage. Finger tightening is sufficient to obtain a good seal. Overtightening can
cause the channels to cut into the membrane.
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MINI PROTEAN II
MULTISCREEN
MINI PROTEAN II
MULTISCREEN