Bio-Rad Mini-PROTEAN II Multiscreen Apparatus User Manual

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OR:
5% BLOTTO in 100 ml of TBS
Add 5% g of BLOTTO to 100 ml of TBS.
Antibody Buffer, 200 ml
1% BSA - TTBS
Add 2 g BSA to 200 ml TTBS. Stir to dissolve.

OR
1% BLOTTO in TTBS.
Add 2 g of BLOTTO to 200 ml of TTBS.

First Antibody Solution

Dilute antigen specific primary antibody to the appropriate titer in antibody buffer.

Second Antibody Solution, 100 ml

Dilute species specific Bio-Rad second antibody enzyme conjugate, 1:3,000 by adding

33 µl of conjugate to 100 ml of antibody buffer.

Consult the Immun-Blot assay kit instruction manual for dilution protocols of the col-

loidal gold conjugates.

Color Development Solution

The specific chemicals and buffers are dependent on the enzyme conjugate being used.
See the Immun-Blot assay kit instruction manual for details on how to make the appro-
priate solution.

Section 6
References

1. Jerome, J. F. and Jaehning, J. A.,

Mol. and Cell Bio., 6, 1633 (1986).

2. Johnson, D. A., et. al., Gene Anal. Tech., 1, 3 (1984).

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