Bio-Rad Bio-Dot® and Bio-Dot SF Microfiltration Apparatus User Manual
Page 3
Table of Contents
Page
Section 1
Introduction ..................................................................................1
1.1
Specifications............................................................................................1
Section 2
Special Handling and Features ..................................................1
2.1
Autoclaving ..............................................................................................1
2.2
Chemical Stability ....................................................................................1
Section 3
Bio-Dot SF Assembly ..................................................................3
3.1
Assembly ..................................................................................................3
3.2
Helpful Hints..............................................................................................5
Section 4
Protein Slot Blotting ....................................................................6
4.1
General Recommendations ......................................................................6
4.2
Immunoassay Procedure ..........................................................................6
Section 5
DNA Slot Blotting ........................................................................8
Section 6
RNA Slot Blotting ........................................................................9
6.1
Alkaline RNA Denaturation and Fixation ..................................................9
6.2
Glyoxal RNA Denaturation and Fixation ..................................................9
Section 7
Hybridization Protocols for Nucleic Acids ..............................11
7.1
Probe Recommendations ......................................................................11
7.2
Hybridization Protocols for DNA or RNA Bound to
Nitrocellulose or Zeta-Probe
®
Membrane ..............................................11
7.3
Hybridization Protocols for RNA Probes ................................................13
7.4
Probe Stripping and Rehybridization ......................................................14
Section 8
Solutions for Protein Applications ..........................................15
8.1
Solutions for Nitrocellulose Membrane ..................................................15
8.2
Solutions for Zeta-Probe Membrane ......................................................16
Section 9
Solutions for Nucleic Acid Applications ..................................17
Section 10
Troubleshooting Guide ..............................................................19
Section 11
References ..................................................................................22
Section 12
Ordering Information ................................................................24