Bio-Rad Criterion™ Blotter - Manual (Page 2)

Table of Contents
Page
Section 1 General Information....................................................................................1
1.1 Introduction ................................................................................................................1
1.2 Specifications .............................................................................................................2
1.3 Safety ..........................................................................................................................2
Section 2 Set Up and Basic Operation........................................................................3
2.1 Preparation for Blotting..............................................................................................3
2.2 Begin Transfer............................................................................................................5
2.3 Acidic Transfers .........................................................................................................6
Section 3 Transfer Conditions .....................................................................................6
3.1 General Guidelines to Transfer Buffers and Running Conditions............................6
3.2 Notes on Electrophoretic Transfer Conditions ..........................................................8
Section 4 Strategies For Optimizing Electro-Elution .............................................11
4.1 Optimizing Protein Transfer ....................................................................................11
4.2 Optimizing DNA and RNA Transfer.......................................................................12
Section 5 Choice of Blotting Membranes.................................................................12
5.1 Protein Blotting Membranes ....................................................................................12
5.2 DNA and RNA Blotting Membranes ......................................................................13
Section 6 Troubleshooting Guide ..............................................................................14
6.1 Electrophoretic Transfer...........................................................................................14
Section 7 Maintenance ...............................................................................................16
Section 8 Product Information..................................................................................16
Section 9 References ...................................................................................................17
Section 10 Warranty.....................................................................................................19