Bio-Rad Criterion™ Blotter - Manual (Page 18)

2. The membrane may have been allowed to dry during handling.
• A completely wet membrane has a gray, translucent appearance. White spots will
form on the surface of the membrane as it dries. Since proteins will not bind to the dry
spots, rewet the membrane with methanol and re-equilibrate in transfer buffer.
Power is too low/high
• Always check the current at the beginning of the run. The current may be too low or
too high for a particular voltage setting. If the buffer is prepared improperly, the
conductivity may be too low or too high, and not enough power will be delivered to
the cell. See the power guidelines for specific applications in Section 3.
• Remake the buffer or alter the voltage (increase or decrease).
• Try changing the intensity of blotting (wire vs. plate electrodes).
Immune-Specific Detection
Overall high background, low signal, or lack of development of positive control.
• Consult instructions for immune detection kit or reagents.
Total Protein Detection
Consult user manual for stain or detection kit.
Section 7
Maintenance
Cleaning: Use mild soap and warm water to clean the electrodes, cassettes, and buffer tank.
Use special care when cleaning the electrode cards or plate electrodes. Avoid stretching or
breaking the platinum wires. Avoid scratching or marring the platinum plate. Do not use abra-
sives or strong detergents. The cathode plate (stainless steel) can be cleaned with a mild abra-
sive to remove salt that may be deposited during normal operation. Rinse the fiber pads under
hot water and then in distilled deionized water.
Chemical compatibility: The Criterion Blotter cell components are not compatible with
chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene,
benzene), or acetone. Use of organic solvents voids all warranties.
Section 8
Product Information
Catalog
Number
Product Description
170-4070 Criterion Blotter–Plate electrodes, includes, Cell assembled with
plate electrodes, lid with cables, 2 Criterion gel holder cassettes,
filter paper pack, fiber pad pack, gel blot assembly tray, sealed ice
cooling unit, manual, roller
170-4071 Criterion Blotter–Wire electrodes, includes, Cell assembled with
wire electrodes, lid with cables, 2 Criterion gel holder cassettes,
filter paper pack, fiber pad pack, gel blot assembly tray, sealed ice
cooling unit, manual, roller
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