Bio-Rad Mini Trans-Blot® Cell - Manual (Page 16)

10 Mini-Trans-Blot Electrophoretic Transfer Cell
Section 3
Transfer Conditions
3.1 General Guide to Transfer Buffers and Running
Conditions
Table 3.1 provides guidelines for power conditions using
different buffers. Power conditions are provided for various
run times. Where multiple conditions are displayed, the
higher the voltage, the less time required for the run.
Always use the blue cooling unit.
Table 3.1. Guide to Buffers and Running Conditions.
Buffer Standard Field High
Intensity Field
Buffer Overnight
Transfer High Intensity Field
1 Hour Transfer
SDS-PAGE Gels Buffer A or B or C Buffer A or B or C
A: 25 mM Tris, pH 8.3, 192
mM glycine, with or without
20% MeOH and .025%–0.1%
SDS 30 V, constant
90 mA 100 V, constant
350 mA
B: 48 mM Tris, pH 9.2, 39 mM
glycine, with or without 20%
MeOH and .025%–0.1% SDS
C: 10 mM NaHCO3, 3 mM
NaCO3, pH 9.9, with or
without 20% MeOH and
.025%–0.1% SDS
DNA and RNA
TAE: 20 mM Tris, pH 7.8,
10 mM 30 V, constant
100 mA 80 V, constant
500 mA
TBE: 50 mM Tris, pH 8.3,
50 mM sodium borate, 1.0
mM EDTA
Native Gels
25 mM Tris, pH 8.3,
92 mM glycine. No methanol. 30 V, constant
90 mA 100 V, constant
350 mA
Isoelectric Focusing, Native
Gels, Basic Proteins, Acid
Urea Gels*
0.7% acetic acid 30 V, constant
100 mA 100 V, constant
350 mA
*Please refer to Section 2.3 before transferring.