Bio-Rad Mini Trans-Blot® Cell - Manual (Page 14)

8 Mini-Trans-Blot Electrophoretic Transfer Cell
6. Add the frozen blue cooling unit. Place in tank and fill
to the “blotting” mark on the tank.
7. Add a standard stir bar to help maintain even buffer
temperature and ion distribution in the tank. Set the
speed as fast as possible to keep ion distribution
even.