Bio-Rad Negative Stains - Manual (Page 7)

Section 4
Product Information
4.1 Copper Stain
161-0470 Copper Stain & Destain Kit for Electrophoresis, includes 125 ml
10x Copper Stain, 125 ml 10x Copper Destain, laminated protocol,
and instruction manual
161-0471 Copper Stain , 10x, 125 ml
161-0734 10x Tris/Glycine (Copper destain) , 10x, 1L
4.2 Related Materials
161-0900 Mini-PROTEAN II Ready Gels , 7.5% single percentage gels,
0.375 M Tris-HCl, 10
161-0901 Mini-PROTEAN II Ready Gels , 12% single percentage gels, 0.375
M Tris-HCl, 10
161-0902 Mini-PROTEAN II Ready Gels , 4 - 15% gradient gels, 0.375 M
Tris-HCl, 10
161-0903 Mini-PROTEAN II Ready Gels , 4 - 20% gradient gels, 0.375 M
Tris-HCl, 10
161-0732 10x Tris/Glycine/SDS , 1L
161-0755 10x Tris/Glycine/SDS , 6 x 1L
161-0757 10x Tris/Glycine , 6 x 1L
For information on the vertical slab cell systems, PROTEAN II xi cells
and the Mini-PROTEAN II cells, consult the catalog or contact your
local Bio-Rad representative.
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6. Can a native gel be stained Yes, but the staining pattern is
with Copper Stain? reversed. In this case, the protein
bands stain an opaque blue-green
while the background remains clear.
However, the effect of staining native
proteins has not been examined.
7. How many gels can be This is dependent on the size of the
stained per kit? staining container. A mini-gel can be
stained in as little as 50 ml of Copper
Stain, providing that the entire gel is
submerged during the staining step.
The limiting factor is the volume of
the stain, not the concentration of
Copper Stain.
3.2 References
1. Lee, C., Levin, A. and Branton, D., Anal. Biochem., 166, 308 (1987).
2. Dzandu, J. K., Johnson, J. F. and Wise, G. E., Anal. Biochem., 174, 157 (1988).
3. Wang, D., Dzandu, J. K., Hussain, M. and Johnson, R. M., Anal. Biochem., 180,
311 (1989).
4. Vos, G. J. and Gardiner, P.R., Parasitology, 100, 93 (1990).
5. Vanfleteren, J. R. and Peeters, K., J. Biochem. Biophys. Methods, 20, 227 (1990).
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